Immobilization of mutated xylanase from Neocallimastix patriciarum in E. coli and application for kraft pulp biobleaching
نویسندگان
چکیده
Abstract As an important enzyme, xylanase is widely used in the food, pulp, and textile industry. Different applications of warrant specific conditions including temperature pH. This study aimed to carry out sodium alginate beads as carrier immobilize previous reported mutated from Neocallimastix patriciarum which expressed E. coli, activity immobilization was elevated about 4% at pH 6 13% 62 °C. Moreover, immobilized retained a greater proportion its than wide type thermostability. These properties suggested that has potential apply biobleaching
منابع مشابه
Application of thermoalkalophilic xylanase from Arthrobacter sp. MTCC 5214 in biobleaching of kraft pulp.
A thermoalkalophilic and cellulase-free xylanase produced from Arthrobacter sp. MTCC 5214 by solid-state fermentation using wheat bran as a carbon source was evaluated for prebleaching of kraft pulp. The UV absorption spectrum of the compounds released by enzyme treatment showed a characteristic peak at 280 nm, indicating the presence of lignin in the released colouring matter. Enzymatic preble...
متن کاملBiobleaching Effects of Crude Xylanase from Streptomyces griseorubens LH-3 on Eucalyptus Kraft Pulp
In this work, a xylanase-producing strain, Streptomyces griseorubens LH-3, was cultured, and the crude xylanase was prepared. Analysis of its enzymatic properties revealed that the crude xylanase possessed good thermal stability at temperatures below 60 o C, exhibited a wide pH range from 4.0 to 9.0, and was cellulase-free. This crude enzyme was used to treat eucalyptus kraft pulp, and the rele...
متن کاملImmobilization of Neocallimastix patriciarum xylanase on artificial oil bodies and statistical optimization of enzyme activity.
A thermally stable and alkalophilic xylanase, XynCDBFV, from Neocallimastix patriciarum was overexpressed in Escherichia coli as a recombinant protein fused to the N-terminus of oleosin, a unique structural protein of seed oil bodies. As a result of the reconstitution of the artificial oil bodies (AOBs), the immobilization of active xylanase was accomplished. Response surface methodology (RSM) ...
متن کاملEnhanced Production of Cellulase-free Xylanase by Alkalophilic Bacillus Subtilis Ash and Its Application in Biobleaching of Kraft Pulp
This paper reports high level production of a cellulase-free xylanase using wheat bran, a cost-effective substrate, under submerged fermentation by alkalophilic Bacillus subtilis ASH. Production of xylanase was observed even at alkaline pH up to 11.0 and temperature 60 °C, although the highest enzyme titer was recorded at neutral pH and 37 °C. The enzyme production under optimized fermentation ...
متن کاملBiobleaching of Bagasse Pulp with Xylanase Enzymes and Hydrogen Peroxide
The effect of the operating conditions (temperature, time, pH, enzyme level and pulp consistency) used in the enzymatic step of a XP (Cartazyme-hydrogen peroxide) sequence for bleaching Kraft pulp from bagasse on various properties of the resulting pulp and paper sheet products was studied. The quality of bagasse used, was examined by its yield, brightness,viscosity and the Kappa number. Finall...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Brazilian Journal of Biology
سال: 2023
ISSN: ['1519-6984', '1678-4375']
DOI: https://doi.org/10.1590/1519-6984.243629